aceA target amplification-free CRISPR biosensor with digital microfluidic processing for microRNA profiling in AI-enhanced pediatric sepsis diagnosis
MicroRNA signatures provide an informative readout of host-response dysregulation in pediatric sepsis, but their clinical translation remains limited by low target abundance and multiplexing requirements. Here, a target amplification-free CRISPR biosensing platform was established. The sensing mechanism couples Cas13a target recognition to promoter release, template reconstruction, and transcription-driven Cas13a reactivation, enabling fluorescence amplification through internal molecular cycling without amplify…
A machine learning model trained on microRNA profiles from an automated CRISPR microfluidic platform separated sepsis from non-sepsis in 252 clinical samples with a test AUC of 0.973.
The disease-progression finding rests on a single representative longitudinal case and is described as preliminary. The authors frame the platform only as having potential for in-vitro diagnosis, not as validated clinical practice.
Evidence
- Peer-reviewedBiosensors and Bioelectronics2026-10-05
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Truvace Impact Record TRV-2026-1844, v1: “A target amplification-free CRISPR biosensor with digital microfluidic processing for microRNA profiling in AI-enhanced pediatric sepsis diagnosis.” Truvace, 2026-10-18. /record/TRV-2026-1844 (accessed at citation time). sha256 4b42353738ba4ee3…
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